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plko 1 teton puro vector  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 teton puro vector
    Plko 1 Teton Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet+plko+1+puro+vector/Tet-pLKO-puro+(Plasmid+%2321915)/pm41708864-575-13-15
    Average 96 stars, based on 706 article reviews
    plko 1 teton puro vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Construct:

    Article Title: RB1 loss triggers dependence on ESRRG in retinoblastoma
    Article Snippet: .. Tetracycline-inducible TET-shRB1 and constitutive shRB1 constructs were created by integrating validated siRNA sequences GAAAGGACATGTGAACTTA ( ) and GAACGATTATCCATTCAAA ( ) targeting human RB1 (shRB1) into TET-pLKO.1-Puro vector (Addgene #21915) and pLKO.1-Puro vector, respectively. .. The plasmids were packaged into lentiviral particles by transient co-transfection into HEK293T cells with pMD2G and psPAX2 packaging plasmids using JetPrime reagent (Polyplus).

    Article Title: RB1 loss triggers dependence on ESRRG in retinoblastoma
    Article Snippet: .. Tetracycline-inducible TET-shRB1 and constitutive shRB1 constructs were created by integrating validated siRNA sequences 5′-GAAAGGACATGTGAACTTA-3′ ( ) and 5′-GAACGATTATCCATTCAAA-3′ ( ) targeting human RB1 (shRB1) into TET-pLKO.1-Puro vector (Addgene #21915) and pLKO.1-Puro vector, respectively. .. The plasmids were packaged into lentiviral particles by transient cotransfection into HEK293T cells with pMD2G and psPAX2 packaging plasmids using jetPRIME reagent (Polyplus).

    shRNA:

    Article Title: NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNA
    Article Snippet: Lentiviral shRNA targeting the anti-codon loop of specific tRNAs, tRNA-Ser-TGA (GGCGATGGACTTGAAATCCATTG) and tRNA-Leu-CAG (GGCGCTGCGTTCAGGTCGCAGTC) was designed and cloned into pLKO.1-Blast backbone vector received from Dr. Keith Mostov’s Lab (Addgene # 26655). pLKO.1-Blast-Scramble (CCTAAGGTTAAGTCGCCCTCG) (Addgene # 26701) was used as non-targeting control. .. For the generation of doxycycline-inducible shRNA targeting human NAT10, shRNAs TRCN0000035699 (sh-NAT10-B4, AATTCAGGATTTAGAGACTGG) and TRCN0000035703 (sh-NAT10-B8, ATAGTCACTGTGTACAATTGC) were cloned into Tet-pLKO.1-puro vector (Addgene # 21915, a gift from Dmitri Wiederschain laboratory). .. Tet-pLKO.1-puro-scramble (Addgene # 47541, a gift from Charles Rudin laboratory) was used as control.

    Article Title: NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNA.
    Article Snippet: Lentiviral shRNA targeting the anti-codon loop of specific tRNAs, tRNASer-TGA (GGCGATGGACTTGAAATCCATTG) and tRNA-Leu-CAG (GGCGCTGCGTTCAGGTCGCAGTC) was designed and cloned into pLKO.1-Blast backbone vector received from Dr. Keith Mostov’s Lab (Addgene # 26655). pLKO.1-Blast-Scramble (CCTAAGGTTAAGTCGCCCTCG) (Addgene # 26701) was used as non-targeting control. .. For the generation of doxycycline-inducible shRNA targeting human NAT10, shRNAs TRCN0000035699 (sh-NAT10-B4, AATTCAGGATTTAGAGACTGG) and TRCN0000035703 (sh-NAT10-B8, ATAGTCACTGTGTACAATTGC) were cloned into Tet-pLKO.1-puro vector (Addgene # 21915, a gift from Dmitri Wiederschain laboratory). .. Tet-pLKO.1-puro-scramble (Addgene # 47541, a gift from Charles Rudin laboratory) was used as control.

    Clone Assay:

    Article Title: NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNA
    Article Snippet: Lentiviral shRNA targeting the anti-codon loop of specific tRNAs, tRNA-Ser-TGA (GGCGATGGACTTGAAATCCATTG) and tRNA-Leu-CAG (GGCGCTGCGTTCAGGTCGCAGTC) was designed and cloned into pLKO.1-Blast backbone vector received from Dr. Keith Mostov’s Lab (Addgene # 26655). pLKO.1-Blast-Scramble (CCTAAGGTTAAGTCGCCCTCG) (Addgene # 26701) was used as non-targeting control. .. For the generation of doxycycline-inducible shRNA targeting human NAT10, shRNAs TRCN0000035699 (sh-NAT10-B4, AATTCAGGATTTAGAGACTGG) and TRCN0000035703 (sh-NAT10-B8, ATAGTCACTGTGTACAATTGC) were cloned into Tet-pLKO.1-puro vector (Addgene # 21915, a gift from Dmitri Wiederschain laboratory). .. Tet-pLKO.1-puro-scramble (Addgene # 47541, a gift from Charles Rudin laboratory) was used as control.

    Article Title: NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNA.
    Article Snippet: Lentiviral shRNA targeting the anti-codon loop of specific tRNAs, tRNASer-TGA (GGCGATGGACTTGAAATCCATTG) and tRNA-Leu-CAG (GGCGCTGCGTTCAGGTCGCAGTC) was designed and cloned into pLKO.1-Blast backbone vector received from Dr. Keith Mostov’s Lab (Addgene # 26655). pLKO.1-Blast-Scramble (CCTAAGGTTAAGTCGCCCTCG) (Addgene # 26701) was used as non-targeting control. .. For the generation of doxycycline-inducible shRNA targeting human NAT10, shRNAs TRCN0000035699 (sh-NAT10-B4, AATTCAGGATTTAGAGACTGG) and TRCN0000035703 (sh-NAT10-B8, ATAGTCACTGTGTACAATTGC) were cloned into Tet-pLKO.1-puro vector (Addgene # 21915, a gift from Dmitri Wiederschain laboratory). .. Tet-pLKO.1-puro-scramble (Addgene # 47541, a gift from Charles Rudin laboratory) was used as control.



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